Buy BHK-21-Klon 13 Lebendkultur | Cell Lines | CLS.shop (2023)

FAQs

What is BHK-21 cell line? ›

The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21. Since this time, this cell line has been a laboratory standard for the growth of countless viruses and the study of many biological processes.

It is concluded that BHK-21 cells in roller bottle (480 cm²) grew well and showed more than 4.7×107 cell count/bottle under optimized conditions such as 10 million seeding cell density, incubation time 60 h, incubation temperature 37°C, 3 rpm, and 100 mL growth medium.

Decant the serum containing medium and gently resuspend the cells in serum-free CD BHK-21 Production Medium at a density of 1 × 106 cells/mL.

Baby hamster kidney (BHK) cells are most commonly used in the production of animal products, the most important of which is the production of a vaccine against Foot-and-Mouth Disease and rabies [8].

The T24 cell line has been established from a highly malignant grade III human urinary bladder carcinoma [20]. This cell line can be easily grown in vitro and has been extensively used to evaluate the therapeutic effects of several anticancer drugs.

Doubling time: 12 hours (PubMed=14207308).

A 1 BHK stands for one standard-sized bedroom, hall, and kitchen. This configuration usually comes in a standard size of 400 – 500 sq. ft.

The average size of a human cell is about 100 μm in diameter.

The average diameters of RT4 and T24 cells in their sus- pension state were measured to be 13.6 ± 1.3 μm (n = 19) and 13.1 ± 2.0 μm (n = 20).

If cells are seeded at too low a density, they will go through a lag phase of growth, grow very slowly, or die out completely.

Why is seeding density important? ›

Seeding the right cell density is necessary for optimum cell growth. Seeding a lower cell density will result in lack of cell to cell interaction and communication. As a result the cells will show slow growth and eventually die.

The cell seeding density (cells/cm²), i.e. the number of cells in 1 cm² of growth surface, allows to make the growth and cell proliferation data homogeneous and independent from the growth vessel used, while the total number of cells depends on the surface on which the cells are grown (trivially the greater the surface ...

The medium for BHK cells is αMEM or DMEM (BioWhittaker). FBS makes the cells grow too fast. Infection with Sindbis viral particles will result in premature cell lysis, leading to low yields of protein expression. Cells are grown in a humidified, 37°C, 5% CO2 incubator.

Chinese hamster ovary cells are a very commonly relied-on component in the antibody production process, given their ability to produce even complex proteins similar to those in humans. Their high tolerance for variations in pH, oxygen levels, temperature or cell density makes them ideal for large-scale manufacturing.

WI-38 cell line is the first human diploid cell line to be used in human vaccine preparation. WI-38 cells were isolated from the lung tissue of a 3-month-old, female, embryo. WI-38 is used in viruscide testing.

Cell Line Description: PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice.

The Cell Line

Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.

A term used to describe cells and tissue that look abnormal under a microscope. High-grade cancer cells tend to grow and spread more quickly than low-grade cancer cells. Cancer grade may be used to help plan treatment and determine prognosis.

Full recovery and establishment of a proliferating cell line may take up to two weeks.

Baby hamster kidney cells (BHK) transfected with mouse LRP exhibited dose-dependent binding of mouse PrPTSE that was blocked by GAG inhibitors (Gauczynski et al., 2006). From: Handbook of Clinical Neurology, 2018.

What is Vero monkey cells? ›

Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted from an African green monkey (Chlorocebus sp.; formerly called Cercopithecus aethiops, this group of monkeys has been split into several different species).

1RK Means. 1RK means only 1 room which combines a bedroom hall and a separate kitchen. There are flats on old type buildings with largest carpet area where 1RK is turned into 1BHK by partitioning.

RHK and BHK difference

Bedroom Hall Kitchen, or BHK as the abbreviation indicates, is a term used to refer to this. BHK comes with a wall that divides the hallway into a room, whereas RHK does not. So it is converted into a bedroom, hallway, and kitchen.

3 BHK stands for an apartment or house that has three bedrooms, a living room or hall, and a kitchen. 7. What does 4 BHK Means? 4 BHK stands for an apartment or house that has four bedrooms, a living room or hall and a kitchen.

If you have a medium-sized window and are looking for a cellular shade that reflects that size, go with 1/2″ or 9/16” cell sizes. These sizes are the most popular because they provide good balance for all sizes of window, all without being too overwhelming. Large windows should be covered with 3/4″ single cell shades.

Select the row or rows that you want to change. On the Home tab, in the Cells group, click Format. Under Cell Size, click Row Height. In the Row height box, type the value that you want, and then click OK.

At 0.1 to 5.0 μm in diameter, prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters ranging from 10 to 100 μm.

The cell lengths were between 14.5 μm and 26.2 μm for MCF10A and between 19.9 μm and 33.9 μm for MCF7 cells.

MCF-7 cells are fairly large adherent cells, with typical cell size measuring 20-25 microns.

How do you remove debris from suspension culture? ›

One of the simplest methods of cell debris removal is density-gradient centrifugation. Density-gradient centrifugation harnesses a device called a centrifuge that spins a heterogenous mixture at high speeds.

Note that most cells must not be split more than 1:10 as the seeding density will be too low for the cells to survive. As a general guide, from a confluent flask of cells: 1:2 split should be 70-80% confluent and ready for an experiment in 1 to 2 days.

Cells may be stressed and undergo cell death due to competition for resources and overcrowding. Generally, you should avoid letting your cells become over confluent because they'll likely start to die off (sometimes very quickly).

Typically, 20,000 – 75,000 cells are seeded per well in Oris™ and Oris™ Pro 96-well plates, and 2,500 – 10,000 per well in Oris™ Pro 384-well plates.

Importance of seeding your torrent files

First, it helps with the speed of download. The more seeders there are on a torrent, the faster the download of the torrent. This happens because you are not downloading the torrent from the actual torrenting site.

Seeding the cells at a heavy density should be avoided as the monolayer will get "sick" (cells starting to become necrotic, sheets of cells peeling away from the monolayer, etc). You could lose your entire monolayer that way.

Cell density should be kept between 2 and 20 × 10⁶/ml during the culture build up.

Depending upon the cell type, suspension cultures are seeded at densities from 2 × 10⁴ to 5 × 10⁵ viable cells/mL and can attain densities of 2 × 10⁶ cell/mL. If cells are seeded at too low a density they will go through a lag phase of growth, grow very slowly, or die out completely.

In general, media should be changed every 2-3 days. However, this will depend on the cell culture type cell density and volume of medium used per unit of surface.

How do you change cell medium? ›

To change media, warm up culture media at 37°C using a water bath or incubator for at least 30 min. Aspirate old media from the flask and replace the media with the necessary volume of fresh pre-warmed culture media and return to incubator.

The most common freezing medium is 90% FBS/10% DMSO. For less finicky cells and for tissue culture on a budget, 10% DMSO in cell growth medium can also be used. After centrifugation, resuspend the cell pellet in 1 mL of freezing medium per cryovial.

Activase is a tissue plasminogen activator for treatment of acute myocardial infarction.

The thrombolytic medication alteplase (Activase), which was approved by the US Food and Drug Administration in 1987, was the first commercially available recombinant protein produced from CHO cells.

HEK293, or human embryonic kidney-derived epithelial cells, are arguably one of the most commonly used cell lines in cell biology research. But why?

HeLa cells are the world's most commonly used human cell lines, and have served as a standard for understanding many fundamental biological processes.

A Master Cell Bank (MCB) is produced from the original therapeutic-producing cell line. It is cryopreserved in multiple vials to prevent genetic variation and potential contamination by eliminating the total number of times a cell line is passaged or handled during the manufacturing process.

Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P.

The 3B-11 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). 3B-11 cells were cloned in 1992 by limiting dilution. They are resistant to lysis by activated macrophages as measured in the chromium release assay.

What is human neuroblastoma cell line? ›

Neuroblastoma (NB) cell lines are transformed, neural crest derived cells, capable of unlimited proliferation in vitro. These cell lines retain the ability of differentiation into neuronal cell types on treatment with various agents.

AML cell lines are obtained from human peripheral blood or bone marrow. After blood/marrow aspiration, the sample is mixed with PBS in a 1:1 or 1:2 ratio and transferred to centrifuge tubes containing gradient buffer.

Tumor cell lines (or cancer cell lines) are derived from the cancerous growths on an organ in a human or animal subject. These cell lines have undergone laboratory modifications that render them immortalized—they are capable of proliferating indefinitely in an in vitro environment.

The Hep-G2/2.2. 15 human hepatoblastoma cell line, a subclone of HepG2 human hepatoblastoma that stably expresses the hepatitis B virus, is one of the most widely used models for HBV-associated liver disease.

3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for 3this specific line of 3T3 cells is used to study adipose tissuerelated diseases and dysfunctions.

Aedes albopictus clone C6/36 is a cell that was isolated from the larva of an Asian tiger mosquito. This cell line was deposited by KH Eckels.

DMEM is not recommended for growing THP-1 cells.

Urine is collected to test for tumor markers produced by a neuroblastoma tumor. A tumor marker is a substance found in higher than normal amounts in the blood, urine, or body tissues of people with certain kinds of cancer. Urinary catecholamine metabolites are found in more the 85% of people with neuroblastoma.

Tumor-derived cell lines have long been used to study the underlying biologic processes in cancer, as well as screening platforms for discovering and evaluating the efficacy of anticancer therapeutics.

The SH-SY5Y cell line is a thrice cloned subline of the neuroblastoma cell line SK-N-SH (ATCC HTB-11), which was established in 1970 from a metastatic bone tumor from a 4-year-old cancer patient. Applications include use as a transfection host or in immunology, neuroscience, and toxicology research.