Terms & Conditions
Thank you for visiting our site. These terms and conditions of use are applicable to the United States, Canada and Puerto Rico websites (“collectively the Web Site”) operated by VWR (the “Company”). If you are accessing the Web Site from outside the United States, Canada, or Puerto Rico, please see the appropriate international website, available at www.vwr.com, for applicable terms and conditions. All users of the Web Site are subject to the following website terms and conditions of use (these “Terms of Use”). Please read these Terms of Use carefully before accessing or using any part of the Web Site. By accessing or using the Web Site, you agree that you have read, understand and agree to be bound by these Terms of Use, as amended from time to time, as well as the Company Privacy Policy, which is hereby incorporated into these Terms of Use. If you do not wish to agree to these Terms of Use, do not access or use any part of the Web Site.
The Company may revise and update these Terms of Use at any time without notice by posting the amended terms to the Web Site. Your continued use of the Web Site means that you accept and agree to the revised Terms of Use. If you disagree with the Terms of Use (as amended from time to time) or are dissatisfied with the Web Site, your sole and exclusive remedy is to discontinue using the Web Site.
Site Use
The information contained on this Web Site is provided for informational purposes only. Although the information is believed to correct at the time of publishing, you should make your own determination as to its suitability for your use. Not all of the products or services described in this Web Site are available in all jurisdictions or to all potential customers, and nothing herein is intended as an offer or solicitation in any jurisdiction or to any potential customer where such offer or sale is not qualified.
Purchase of Products and Services
These Terms and Conditions apply only to the use of the Web Site. Please note, the terms and conditions relating to service, product sales, promotions, and other related activities can be found at https://us.vwr.com/store/content/externalContentPage.jsp?path=/en_US/about_vwr_terms_and_conditions.jsp, and those terms and conditions control any purchases of products or services from the Company.
Interactive Features
The Web Site may contain bulletin board services, chat areas, news groups, forums, communities, personal web pages, calendars, and/or other message or communication facilities designed to enable you to communicate with the public at large or with a group (collectively, "Community Feature"). You agree to use the Community Feature only to post, send and receive messages and material that are proper and related to the particular Community Feature. You agree to use the Web Site only for lawful purposes.
A. Specifically you agree not to do any of the following when using a Community Feature:
1. Defame, abuse, harass, stalk, threaten or otherwise violate the legal rights (such as rights of privacy and publicity) of others.
2. Publish, post, upload, distribute or disseminate any inappropriate, profane, defamatory, infringing, obscene, indecent or unlawful topic, name, material or information.
3. Upload files that contain software or other material protected by intellectual property laws (or by rights of privacy of publicity) unless you own or control the rights thereto or have received all necessary consents.
4. Upload files that contain viruses, corrupted files, or any other similar software or programs that may damage the operation of another's computer.
5. Intercept or attempt to intercept electronic mail not intended for you.
6. Advertise or offer to sell or buy any goods or services for any business purpose, unless such Community Feature specifically allows such messages.
7. Conduct or forward surveys, contests, pyramid schemes or chain letters.
8. Download any file posted by another user of a Community Feature that you know, or reasonably should know, cannot be legally distributed in such manner or that you have a contractual obligation to keep confidential (notwithstanding its availability on the Web Site).
9. Falsify or delete any author attributions, legal or other proper notices or proprietary designations or labels of the origin or source of software or other material contained in a file that is uploaded.
10. Misrepresent an affiliation with any person or organization.
11. Engage in any other conduct that restricts or inhibits anyone’s use of the Web Site, or which, as determined by the Company, may harm the Company or users of the Web Site or expose them to liability.
12. Violate any applicable laws or regulations or violate any code of conduct or other guidelines which may be applicable for any particular Community Feature .
13. Harvest or otherwise collect information about others, including e-mail addresses, without their consent.
B. You understand and acknowledge that you are responsible for whatever content you submit, you, not the Company, have full responsibility for such content, including its legality, reliability and appropriateness. If you post in the name of or on behalf of your employer or another entity, you represent and warrant that you are authorized to do so. By uploading or otherwise transmitting material to any area of the Web Site, you warrant that the material is your own or is in the public domain or otherwise free of proprietary or other restrictions and that you have the right to post it to the Web Site. Additionally, by uploading or otherwise transmitting material to any area of the Web Site you are granting the Company a irrevocable, royalty-free, worldwide right to publish, reproduce, use, adapt, edit and/or modify such material in any way, in any and all media now known or hereafter discovered, worldwide, including on the Internet and World Wide Web, for advertising, commercial, trade and promotional purposes, without additional limitation or compensation, unless prohibited by law, and without notice, review or approval.
C. The Company reserves the right, but does not assume any responsibility, to (1) remove any material posted on the Web Site which the Company, in its sole discretion, deems inconsistent with the foregoing commitments, or otherwise inappropriate for any reason; and (2) terminate any user’s access to all or part of the Web Site. However, the Company can neither review all material before it is posted on the Web Site nor ensure prompt removal of objectionable material after it has been posted. Accordingly, the Company assumes no liability for any action or inaction regarding transmissions, communications or content provided by third parties. The Company reserves the right to take any action it deems necessary to protect the personal safety of users of this Web Site and the public; however, the Company has no liability or responsibility to anyone for performance or nonperformance of the activities described in this paragraph.
D. Your failure to comply with the provisions of (A) or (B) above may result in the termination of your access to the Web Site and may expose you to civil and/or criminal liability.
Special note about Community Feature Content
Any content and/or opinions uploaded, expressed or submitted through any Community Feature or any other publicly available section of the Web Site (including password-protected areas), and all articles and responses to questions, other than the content explicitly authorized by the Company, are solely the opinions and responsibility of the person or entity submitting them and do not necessarily reflect the opinions of the Company. By way of example, any recommended or suggested use of products or services available from the Company that is posted through a Community Feature is not a sign of approval or recommendation by the Company. If you choose to follow any such recommendation you do so at your own risk.
Links to Third Party Sites
The Web Site may contain links to other websites on the internet. The Company is not responsible for the content, products, services or practices of any third party websites, including without limitation sites linked to or from the Web Site, sites framed within the Web Site or third party advertisements, and does not make representations regarding their quality, content or accuracy. The presence of links from the Web Site to any third party website does not mean that we approve of, endorse or recommend that website. We disclaim all warranties, express or implied, as to the accuracy, legality, reliability or validity of any content on any third party website. Your use of third party websites is at your own risk and subject to the terms and conditions of use for such websites.
Proprietary Rights to Content
You acknowledge and agree that the entire contents of the Web Site (including all information, data, software, graphics, text, images, logos, and/or other material) and the design, selection, collection, arrangement and assembly thereof, are proprietary to the Company and are protected by United States and international intellectual property laws. You are authorized only to use the content on the Web Site for personal use or legitimate business purposes. You may not copy, modify, create derivative works of, publicly display or perform, republish, store, transmit, distribute, remove, delete, augment, add to, participate in the transfer of, license or sell any of the material on the Web Site without the prior written consent of the Company, except to: (a) store copies of such materials temporarily in RAM, (b) store files that are automatically cached by your Web browser for display enhancement purposes, and (c) print a reasonable number of pages of a Web Site; provided in each case that you do not alter or remove any copyright or other proprietary notices included in such materials. Neither the title nor any intellectual property rights to any information or material on the Web Site are transferred to you, but remain with the Company or the applicable owner of such content.
Trademarks
The Company name and logo, and all related names, logos, product and service names appearing on the Web Site are trademarks of the Company and/or the applicable third party suppliers. They may not be used or redisplayed without the Company’s prior written consent.
Disclaimer of Liability
The Company does not assume any liability for the materials, information and opinions provided on, or available through, the Web Site (the “Site Content”). Reliance on the Site Content is solely at your own risk. The Company disclaims any liability for injury or damages resulting from the use of any Site Content.
THE WEB SITE, THE SITE CONTENT AND THE PRODUCTS AND SERVICES PROVIDED ON OR AVAILABLE THROUGH THE WEB SITE ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS, WITH ALL FAULTS. NEITHER THE COMPANY NOR ANY PERSON ASSOCIATED WITH THE COMPANY MAKES ANY WARRANTY OR REPRESENTATION WITH RESPECT TO THE QUALITY, ACCURACY, OR AVAILABILITY OF THE WEB SITE. SPECIFICALLY, BUT WITHOUT LIMITING THE FOREGOING, NEITHER THE COMPANY NOR ANYONE ASSOCIATED WITH THE COMPANY WARRANTS OR REPRESENTS THAT THE WEB SITE, THE SITE CONTENT OR THE SERVICES PROVIDED ON OR THROUGH THE WEB SITE WILL BE ACCURATE, RELIABLE, ERROR-FREE OR UNINTERRUPTED; THAT DEFECTS WILL BE CORRECTED; THAT THE WEB SITE OR THE SERVER THAT MAKES IT AVAILABLE ARE FREE OF VIRUSES OR OTHER HARMFUL COMPONENTS; OR THAT THE WEB SITE WILL OTHERWISE MEET YOUR NEEDS OR EXPECTATIONS. THE COMPANY DISCLAIMS ALL WARRANTIES OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NON-INFRINGEMENT.
IN NO EVENT WILL THE COMPANY OR ITS LICENSORS OR CONTRACTORS BE LIABLE FOR ANY DAMAGES OF ANY KIND, UNDER ANY LEGAL THEORY, ARISING OUT OF OR IN CONNECTION WITH YOUR USE OF, OR INABILITY TO USE, THE WEB SITE, THE SITE CONTENT, ANY SERVICES PROVIDED ON OR THROUGH THE WEB SITE OR ANY LINKED SITE, INCLUDING ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, CONSEQUENTIAL OR PUNITIVE DAMAGES, INCLUDING, BUT NOT LIMITED TO, PERSONAL INJURY, LOST PROFITS OR DAMAGES RESULTING FROM DELAY, INTERRUPTION IN SERVICE, VIRUSES, DELETION OF FILES OR ELECTRONIC COMMUNICATIONS, OR ERRORS, OMISSIONS OR OTHER INACCURACIES IN THE WEB SITE OR THE SITE CONTENT OR SERVICES, WHETHER OR NOT THERE IS NEGLIGENCE BY THE COMPANY AND WHETHER OR NOT THE COMPANY HAS BEEN ADVISED OF THE POSSIBILITY OF ANY SUCH DAMAGES, UNLESS PROHIBITED BY APPLICABLE LAW.
Indemnification
You agree to indemnify and hold harmless the Company and its officers, directors, agents, employees, and others involved in the Web Site, from and against any and all liabilities, expenses, damages and costs, including reasonable attorneys’ fees, arising from any violation by you of these Terms and Conditions of Use, your use of the Web Site or any products, services or information obtained from or through the Web Site, your connection to the Web Site, any content you submit on the Web Site through any Community Feature , or your violation of any rights of another.
Applicable Law; International Use
These terms will be governed by and construed in accordance with the laws of the State of Pennsylvania, without regard to any principles of conflicts of law. You agree that any action at law or in equity that arises out of or relates to these Terms and Conditions of Use will be filed exclusively in the state or federal courts located in Pennsylvania and you hereby consent and submit to the personal jurisdiction of such courts for the purposes of litigating any such action.
These Terms and Conditions of Use are applicable to users in the United States, Canada, and Puerto Rico. If you are accessing the Web Site from outside the United States, Canada, or Puerto Rico, please see the appropriate international website, available at www.vwr.com, for applicable terms and conditions. If you elect to access this Web Site from outside of the specified jurisdictions rather than use the available international sites, you agree to these Terms and Conditions of Use and that such terms will be governed and construed with the laws of the United States and the State of Pennsylvania and that we make no representation that the materials or services on this Web Site are appropriate or available for use in those other jurisdictions. In any event, all users are responsible for their own compliance with local laws.
General Terms
These Terms and Conditions of Use, as they may be amended from time to time, constitute the entire agreement and understanding between you and us governing your use of the Web Site. Our failure to exercise or enforce any right or provision of the Terms and Conditions of Use shall not constitute a waiver of such right or provision. If any provision of the Terms and Conditions of Use is found by a court of competent jurisdiction to be invalid, you nevertheless agree that the court should endeavor to give effect to the parties' intentions as reflected in the provision, and the other provisions of the Terms and Conditions of Use shall remain in full force and effect. Neither a course of dealing or conduct between you and the Company nor any trade practices shall be deemed to modify these Terms and Conditions of Use. You agree that regardless of any statute or law to the contrary, any claim or cause of action arising out of or related to use of the Site or the Terms and Conditions of Use must be filed within one (1) year after such claim or cause of action arose or be forever barred. Any rights not expressly granted herein are reserved by and for the Company. We may terminate your access, or suspend any user's access to all or part of the Site, without notice, for any conduct that we, in our sole discretion, believe is in violation of any applicable law or is harmful to the interests of another user, a third-party provider, a service provider, or us. Any inquiries concerning these Terms and Conditions of Use should be directed to solutions@vwr.com.
Copyright Complaints
We respect the intellectual property of others, and we ask our users to do the same. If you believe that your work has been copied and is accessible on the Site in a way that constitutes copyright infringement, you may notify us by providing our copyright agent the following information:
an electronic or physical signature of the person authorized to act on behalf of the owner of the copyright interest;
a description of the copyrighted work that your claim has been infringed;
identification of the URL or other specific location on the Site where the material that you claim is infringing is located;
your address, telephone number, and e-mail address;
(Video) Cat Burns Shops Collab Demonstration | Full demoa statement by you that you have a good faith belief that the disputed use is not authorized by the copyright owner, its agent, or the law; and
a statement by you, made under penalty of perjury, that the above information in your notice is accurate and that you are the copyright owner or authorized to act on the copyright owner's behalf.
Our agent for notice of claims of copyright infringement on the Site can be reached at: solutions@vwr.com.
FAQs
What is the corning mem minimum essential medium? ›
Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation.
What is minimum essential medium used for? ›Minimal Essential Medium (MEM) is a synthetic cell culture medium developed by Harry Eagle first published in 1959 in Science that can be used to maintain cells in tissue culture.
What are the components of minimum essential medium? ›Eagle's minimum essential medium modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.
What is the full form of MEM media? ›MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media.
Are emem and MEM the same? ›A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Can I use DMEM instead of MEM? ›DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM. You should also consider Opti-MEM which, in my experience, is even better than DMEM.
What does MEM contain? ›MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid.
What is the difference between EMEM and MEM alpha? ›Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations.
What is the difference between DMEM and EMEM? ›Both mediums lack protein and contain the amino acids, salts, glucose and vitamins, required to provide a cell with energy and maintain cells in tissue culture, but the DMEM formulation is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM.
What is minimum essential value? ›Minimum Essential Coverage (MEC) and Minimum Value (MV): Minimum Essential Coverage is a lower threshold than Minimum Value (MV). MV is the 60% Actuarial Value and is met when a plan pays on average at least 60% of the actuarial value of allowed benefits under the plan.
What is minimum essential product? ›
A minimum viable product, or MVP, is a product with enough features to attract early-adopter customers and validate a product idea early in the product development cycle. In industries such as software, the MVP can help the product team receive user feedback as quickly as possible to iterate and improve the product.
What are the 5 main components of cell culture base media? ›Essential components of cell culture media include carbohydrates, amino acids, vitamins, minerals, and a pH buffer system. Your choice of media can affect the accuracy, reproducibility, and relevance of your experimental results.
What does MEM mean in technology? ›Microsoft Endpoint Manager (MEM) is a cloud-based solution that is designed to address the challenges associated with deploying, managing and securing devices in the enterprise. This includes servers, PCs and mobile devices.
What does MEM stand for in electronics? ›MEMS (Microelectromechanical systems) is the technology of microscopic devices incorporating both electronic and moving parts.
Does MEM stand for memory? ›MEM stands for Memory
This definition appears very frequently and is found in the following Acronym Finder categories: Information technology (IT) and computers.
MEM, no glutamine, no phenol red.
What is the composition of MEM in Atcc? ›ATCC's modified MEM medium contains non-essential amino acids, sodium pyruvate, and reduces the amount of sodium bicarbonate. This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors.
How much glucose is in EMEM? ›Glucose concentration is 1000 mg/L in EMEM.
What is the most popular cell culture media? ›MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media.
What is the best media for HEK293 cells? ›Growth Medium: To keep HEK293 cells happy, use a high-glucose growth media such as Eagle's Minimum Essential Medium (EMEM) or Dulbecco's Modified Eagle's Medium (DMEM), supplemented with Fetal Bovine Serum (FBS) to a final concentration of 10%. Growth media should be replaced every 2-3 days.
What filter to use for cell culture medium? ›
Essential Stericup® and sustainable Stericup® E filters are integral devices for filtering media to protect cell cultures. Choose from membranes and pore sizes for fast flow, low protein binding, mycoplasma exclusion, and plastic waste reduction.
Does MEM have glucose? ›MEM, high glucose, high sodium bicarbonate.
Does MEM contain calcium? ›Minimum Essential Medium Eagle (MEM) (Joklik Modification) (For Suspension Culture) w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate. AT079 is Joklik modification of Minimum Essential Medium. This medium is designed for use with suspension cells, hence does not contain calcium.
What is MEM supplement? ›Gibco™ MEM Vitamin Solution is used as a growth supplement for cell culture medium, to increase cell growth and viability.
What is alpha MEM and minimum essential medium? ›MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts.
What is MEM non-essential amino acids? ›The MEM NEAA are used as an additional amino acid pool for rapidly proliferating cells or for cells, which have lost the ability to synthesize non-essential amino acids on their own. The non-essential amino acids include glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid , L-proline, and L-serine.
What is the composition of DMEM medium? ›Dulbecco's Modified Eagle's Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.
What is the difference between Eagle's minimal essential medium and DMEM? ›DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Why is DMEM media pink? ›If the pH value is 7.4, the culture medium will be red. With 40 µM of phenol red in DMEM, the medium changes color when the pH value rises, turning pink at a pH value of 7.6 and purple at 7.8. This might mean that the medium is contaminated with yeast or a fungus.
Can you use RPMI instead of DMEM? ›DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
What is the difference between MEC and MV? ›
Minimum Essential Coverage is also different than Minimum Value (MV). The Minimum Value refers to the comprehensiveness that a plan offers. An MEC, for instance, has a MV of 60 percent, which is the Actuarial Value.
How do you choose a minimum value? ›You can find this minimum value by graphing the function or by using one of the two equations. If you have the equation in the form of y = ax^2 + bx + c, then you can find the minimum value using the equation min = c - b^2/4a.
Do MEC plans meet minimum value? ›Minimum value has a higher threshold for coverage than MEC. In other words, a MEC plan can meet ACA requirements even if it doesn't meet minimum value standards. However, if an employer's offered plan doesn't provide minimum value, the employee may qualify for a premium tax credit in the Marketplace.
What is the difference between MVP and MMP? ›Despite their similar acronyms, there are differences. At the simplest level, you can say that an MVP is about testing out the product idea to see if it is fit for market; and an MMP is about getting a user-ready version of the product to market as quickly as possible.
What is next after MMP? ›Minimum Marketable Release (MMR): The version that MMP evolves into and gets released into the market. Minimum Marketable Feature (MMF): The crucial features of the product that have the potential to be released as an individual product.
What is the main difference between MVP and MLP? ›What are MVP and MLP? MVP — the minimum viable product is a product with a minimum set of basic features that is used to collect feedback for further iterations. MLP — the minimum loveable product that has not only basic functionality, but also the potential to make users fall in love with it.
What are the 3 types of cell culture? ›Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.
What vitamins are needed for plant tissue culture? ›The vitamins most frequently used in cell and tissue culture media include thiamin (B1), nicotinic acid, pyridoxine (B6), and myo-inositol. Thiamin is the one vitamin that is basically required by all cells for growth. Thiamin is normally used at concentrations ranging from 0.1 to 10.0 mg/liter.
What are the six types of culture media? ›These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media.
What are word examples of mem? ›-mem- comes from Latin, where it has the meaning "mind; memory. '' This meaning is found in such words as: commemorate, immemorial, memento, memo, memoir, memorabilia, memorandum, memorial, memorize, memory, remember, remembrance.
What is mem radio? ›
Memory Radio features specially selected content from the BBC Radio Archives, decade by decade, to create an audio experience for people living with dementia. Currently, there are three 90 minutes programmes covering the 1940s, 1950s and 1960s.
What type of word is mem? ›As detailed above, 'mem' is a noun.
What does MEM mean in mechanical engineering? ›Master of Engineering Management (MEM) (or Master of Science in Engineering Management (MSEM)) is a professional master's degree that bridges the gap between the field of engineering or technology and the field of business management.
What company is MEM? ›MEM Company, Inc. manufactures, sells, and distributes various men's, women's, and children's toiletries, fragrances, wallets, and accessories. The Company serves customers in the United States.
What is the difference between CPU and MEM? ›Processor: RAM stands for Random Access Memory, and is used as a short-term memory storage space. The processor, also known as the CPU, provides the instructions and processing power the computer needs to do its work.
What is CPU and MEM? ›CPU stands for the central processing unit. RAM stands for random access memory. CPU and RAM are semiconductors that use integrated circuits to store and move data. The processor uses RAM to store data it uses temporarily. RAM is connected to the CPU via the front-side (FSB) or system bus.
What is mem in android? ›A managed memory environment, like the ART or Dalvik virtual machine, keeps track of each memory allocation. Once it determines that a piece of memory is no longer being used by the program, it frees it back to the heap, without any intervention from the programmer.
What is the difference between MEM and DMEM? ›The major differences between DMEM and MEM include the use of lower concentrations of amino acids, vitamins, and inorganic phosphate and the inclusion of lactate and glutamine in the medium.
What is the difference between DMEM and alpha MEM? ›Background: Alpha modified Eagle's minimum essential medium (α-MEM) and Dulbecco's modified Eagle's medium (DMEM) are the basic media for cell culture. α-MEM has minimum glucose and amino acid concentration than DMEM.
Can you use DMEM for suspension cells? ›Dulbecco's Modified Eagle Medium (DMEM) can be used to culture a variety of suspension and adherent mammalian cells, including primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What are the three types of cell culture media? ›
Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.
Is DMEM made from Eagles? ›DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells.
Is DMEM a complete medium? ›DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.
What is the difference between advanced DMEM and DMEM? ›Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology.
What is Alpha MEM used for? ›MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
Why is DMEM used in cell culture? ›Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is the difference between MVP and MLP? ›What are MVP and MLP? MVP — the minimum viable product is a product with a minimum set of basic features that is used to collect feedback for further iterations. MLP — the minimum loveable product that has not only basic functionality, but also the potential to make users fall in love with it.
What is the difference between MVP and MDP? ›Minimum Desirable Product (MDP)
Excluding users from MVP decisions, leaves products and services in danger of poor user adoption, MVP or not. Often MVP's focus on usability fixes. Instead of strategic pivots, they end up being tactical-- for users-- though oftentimes very strategic for the business.