IMR-90 - CCL-186 | ATCC (2023)

FAQs

What is the use of IMR 90 cell line? ›

IMR-90 cells are reported to be capable of attaining 58 population doublings before the onset of senescence. The cell line may be used as an alternate to WI-38 and other human lung cell strains, considering division potential and viral susceptibilities.

Human primary fibroblasts (IMR90, ATCC #CCL-186) • Doubling time: +/- 30-40hrs (at 3% O2) • Medium: DMEM, high glucose, + glutamine, w/o pyruvate, Pen/Strep, 10% FBS (change media every three days). All primary human fibroblasts lose telomeric DNA with every doubling, and so will eventually senesce.

Primary Lung Fibroblast, Normal, Human (HLF) is a cell line that's an ideal culture model for potential diagnostic methods for the early detection of lung cancer cells, reconstruction studies, and advancement of cancer research.

Human Fetal Lung Fibroblast Cells (MRC-5 Line) The MRC-5 cell line is commonly utilized in vaccine development, as a transfection host in virology research, and for in vitro cytotoxicity testing.

Immortal cell lines are often used in research in place of primary cells. They offer several advantages, such as they are cost effective, easy to use, provide an unlimited supply of material and bypass ethical concerns associated with the use of animal and human tissue.

HeLa cells are the world's most commonly used human cell lines, and have served as a standard for understanding many fundamental biological processes.

The growth rate equals 0.01356 cells per hour.

Divide the elapsed time in hours by the number of generations that passed during that time. For example, two hours divided by four generations equals 0.5 hours per generation. Multiply the result by 60 to convert to minutes per generation. In the example, the doubling time is 0.5 * 60, or 30 minutes.

The formula for doubling time is: Doubling time = ln(2) / (growth rate), where “ln” represents the natural logarithm and the growth rate is expressed as a decimal or percentage.

These findings indicate that fibroblast cell lines immortalized with transforming genes of human papillomavirus retain complete permissiveness to HCMV infection and support plaque formation. The IF cell line will be useful for future genetic analysis of HCMV.

What is normal human fibroblast cell line? ›

Primary Dermal Fibroblast; Normal, Human, Adult (HDFa) is a skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery, and skin diseases, including scleroderma.

For primary human fibroblasts, the cell cycle lasts between 16 and 28 hours with a mean of 20 hours.

Fibroblasts are originally derived from primitive mesenchyme and therefore display the filament protein vimentin, which acts as a marker of mesodermal origin. In some cases, epithelial cells may also produce fibroblasts, a process which is referred to as epithelial–mesenchymal transition (EMT).

We identified CD248 and ITGA8 as cell surface markers for human lung fibroblast subtypes. Given their localization, two major subtypes, CD248^highITGA8^low human fibroblast-like cells and CD248^lowITGA8^high human fibroblast-like cells, appear to be associated with collagen fibers and elastic fibers, respectively.

In the lung, fibroblasts reside enmeshed in ECM within the interstitial space until they are required for wound repair.

The major disadvantage to using immortalized cells is that these cells cannot be considered normal, in that they divide indefinitely and sometimes express unique gene patterns not found in any cell type in vivo. Therefore, they might not have the relevant attributes or functions of normal cells.

Although many animal cells stop dividing after a finite number of cell divisions, cells that have been immortalized through spontaneous mutations or genetic manipulation can be maintained indefinitely in cell lines.

Among the important scientific discoveries of the last century was the first immortal human cell line known as “HeLa” — a remarkably durable and prolific line of cells obtained during the treatment of Henrietta's cancer by Johns Hopkins researcher Dr. George Gey in 1951.

The HeLa cell line is the oldest, most widely distributed, permanent human cell line.

High Five (BTI-Tn-5B1-4) is an insect cell line that originated from the ovarian cells of the cabbage looper, Trichoplusia ni. It was developed by the Boyce Thompson Institute for Plant Research.

What has the oldest cell line? ›

HeLa (/ˈhiːlɑː/; also Hela or hela) is an immortalized cell line used in scientific research. It is the oldest and most commonly used human cell line.

The rule of 70 is used to determine the number of years it takes for a variable to double by dividing the number 70 by the variable's growth rate. The rule of 70 is generally used to determine how long it would take for an investment to double given the annual rate of return.

For the first 12 hours after conception, the fertilized egg remains a single cell. After 30 hours or so, it divides from one cell into two. Some 15 hours later, the two cells divide to become four.

The reason why the rule of 70 is popular in finance is because it offers a simple way to manage complicated exponential growth. It breaks down growth formulas into a simple equation using the number 70 alongside the rate of return.

Doubling time: ~24 hours (CLS=500142); ~25-30 hours (DSMZ=ACC-550).

What is the Rule of 72? The Rule of 72 is a calculation that estimates the number of years it takes to double your money at a specified rate of return. If, for example, your account earns 4 percent, divide 72 by 4 to get the number of years it will take for your money to double. In this case, 18 years.

The doubling time is the time it takes your cells to double in number. It is useful to know the doubling time of your cells so that you can plate the appropriate number for transduction with a lentiviral library or construct.

Let's discuss the formula for doubling numbers. We can double any number in two ways. 1) Multiply the number by 2. 2) Add the number to itself.

The doubling time of a population exhibiting exponential growth is the time required for a population to double. Implicit in this definition is the fact that, no matter when you start measuring, the population will always take the same amount of time to double.

The doubling time is the time it takes for a population to double in size/value. It is applied to population growth, inflation, resource extraction, consumption of goods, compound interest, the volume of malignant tumours, and many other things that tend to grow over time.

What cell lines for virus growth? ›

Diploid cell lines

The cells retain their diploid character, hence the name 'diploid cell line'. A cell line of this kind is susceptible to a broad spectrum of different kinds of viruses. Diploid cells are used for the cultivation of viruses from patients and also for the production of some live virus vaccines.

Gibco Viral Production Cells are a derivative of the HEK 293F cell line and are a core component of the LV-MAX Lentiviral Production System. Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium.

For accurate identification of viruses, different types of cell lines should be prepared to inoculate the suspected sample. The most important cell lines widely used for viral diagnosis are primary rhesus monkey kidney cells (RhMK), primary rabbit kidney cells, MRC-5, human foreskin fibroblasts, HEp-2, and A549.

Cancer-associated fibroblasts are important players of the tumour microenvironment. They influence numerous processes during tumour development and progression, including the response of cancer cells to treatment. As a consequence, this cell type has emerged has a prominent target in anti-cancer therapy.

With the exception of the smallest group of fibroblasts (fractions 25–30), the diameter of which approached that of bare nuclei (7.3 ± 0.6-μm nuclear diameter vs. 8.44 ± 2.5-μm cell diameter), the median FALS value correlated well with average cell volume (r = 0.94).

Besides their role as inflammatory cells, the fibroblastic stromal cells may play a critical role in enabling chronic inflammation in tissues, where their production of chemokines and growth factors may be critical to perpetuating the recruitment, retention, and survival of leukocytes.

During the aging process, the proliferative and metabolic activity of fibroblasts decreases and their functions are impaired, leading to reduction of the synthesis of structural substances such as collagen, elastin, hyaluronic acid, and chondroitin.

The Hayflick Limit is a concept that helps to explain the mechanisms behind cellular aging. The concept states that a normal human cell can only replicate and divide forty to sixty times before it cannot divide anymore, and will break down by programmed cell death or apoptosis.

Fibroblasts are classically associated to fibrosis and tissue repair, and seldom to regeneration. However, accumulating evidence supports a pro-regenerative role of fibroblasts in different organs.

As a cryoprotective agent, DMSO is added to cell freezing medium to prevent the formation of ice crystals during the freezing process, otherwise cells would be destroyed and dead. It is commonly used in cell banking. DMSO has a low toxicity to cells.

Why is MCF 7 cell line used? ›

MCF7 are primarily used as an in vitro model to study breast cancer biology. Due to the number of variants available, it has applications in development of chemotherapeutic drugs and understanding drug resistance. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen.

African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses.

To produce viral vaccines, candidate vaccine viruses are grown in mammalian, avian or insect tissue culture of cells with a finite lifespan. These cells are typically Madin-Darby Canine Kidney cells, but others are also used including monkey cell lines pMK and Vero and human cell lines HEK 293, MRC 5, Per.

1A, DMSO showed a dose dependent effect on cell proliferation; cells treated with 1.5% DMSO showed an approximately 10% reduction in cell growth.

Research has reported that adding 10% Ficoll 70 to the 10% DMSO containing cryoprotectant makes cells frozen at -80°C for one year without loss of viability, compared with liquid nitrogen storage.

In addition to increased ROS production, decreased cell viability and mitochondrial dysfunction may impair glutamate transporter synthesis by astrocytes. High concentration of DMSO has been shown to degrade membrane structure and disturb secondary protein structures within membrane proteins [26], [27].

The Cell Line

Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.

CO₂ is not a metabolic requirement for cell cultures, its purpose is to dissolve into cell culture medium where a small proportion of it reacts with water to form carbonic acid which in turn interacts with its conjugate base (the dissolved bicarbonate ions in the medium) to control a stable physiological pH through the ...

MCF-7 is a breast cancer cell line with overexpression of estrogen receptor, while MCF 10A is a non-tumorigenic epithelial breast cell line.

The 3T3-L1 murine preadipocyte cell line is a commonly used tool for analysis of the subcellular pathways involved in preadipocytic cell differentiation (a process also commonly known as adipogenesis). The major characteristic of adipogenesis is the intracellular accumulation of membrane-bound lipid droplets.

Why use A549 cell line? ›

The A549 cells have been used to model the alveolar Type II pulmonary epithelium. Studies have shown that this can be particularly useful in research for studying the metabolic processing of lung tissue and for identifying mechanisms of drug delivery to the tissue.

SV40 MES 13 is a mesangial cell line that was isolated in from the glomerulus of a 7-to-10-week-old mouse. This cell line was deposited by LJ Striker.

The Rhesus monkey cell was the initial cell-of-choice to measure the quantity of antibody developed in response to the poliovirus infection.

The mRNA is broken down quickly by the body. It never enters the nucleus, and cannot affect or combine with our DNA in any way to change our genetic code. Instead, COVID-19 mRNA vaccines teach the cell how to make a protein that triggers an immune response specific to COVID-19.

The cell-based vaccine manufacturing process uses mammalian cells (Madin-Darby Canine Kidney, or MDCK cells) to grow flu viruses instead of fertilized hen's eggs.