Terms & Conditions
Thank you for visiting our site. These terms and conditions of use are applicable to the United States, Canada and Puerto Rico websites (“collectively the Web Site”) operated by VWR (the “Company”). If you are accessing the Web Site from outside the United States, Canada, or Puerto Rico, please see the appropriate international website, available at www.vwr.com, for applicable terms and conditions. All users of the Web Site are subject to the following website terms and conditions of use (these “Terms of Use”). Please read these Terms of Use carefully before accessing or using any part of the Web Site. By accessing or using the Web Site, you agree that you have read, understand and agree to be bound by these Terms of Use, as amended from time to time, as well as the Company Privacy Policy, which is hereby incorporated into these Terms of Use. If you do not wish to agree to these Terms of Use, do not access or use any part of the Web Site.
The Company may revise and update these Terms of Use at any time without notice by posting the amended terms to the Web Site. Your continued use of the Web Site means that you accept and agree to the revised Terms of Use. If you disagree with the Terms of Use (as amended from time to time) or are dissatisfied with the Web Site, your sole and exclusive remedy is to discontinue using the Web Site.
Site Use
The information contained on this Web Site is provided for informational purposes only. Although the information is believed to correct at the time of publishing, you should make your own determination as to its suitability for your use. Not all of the products or services described in this Web Site are available in all jurisdictions or to all potential customers, and nothing herein is intended as an offer or solicitation in any jurisdiction or to any potential customer where such offer or sale is not qualified.
Purchase of Products and Services
These Terms and Conditions apply only to the use of the Web Site. Please note, the terms and conditions relating to service, product sales, promotions, and other related activities can be found at https://us.vwr.com/store/content/externalContentPage.jsp?path=/en_US/about_vwr_terms_and_conditions.jsp, and those terms and conditions control any purchases of products or services from the Company.
Interactive Features
The Web Site may contain bulletin board services, chat areas, news groups, forums, communities, personal web pages, calendars, and/or other message or communication facilities designed to enable you to communicate with the public at large or with a group (collectively, "Community Feature"). You agree to use the Community Feature only to post, send and receive messages and material that are proper and related to the particular Community Feature. You agree to use the Web Site only for lawful purposes.
A. Specifically you agree not to do any of the following when using a Community Feature:
1. Defame, abuse, harass, stalk, threaten or otherwise violate the legal rights (such as rights of privacy and publicity) of others.
2. Publish, post, upload, distribute or disseminate any inappropriate, profane, defamatory, infringing, obscene, indecent or unlawful topic, name, material or information.
3. Upload files that contain software or other material protected by intellectual property laws (or by rights of privacy of publicity) unless you own or control the rights thereto or have received all necessary consents.
4. Upload files that contain viruses, corrupted files, or any other similar software or programs that may damage the operation of another's computer.
5. Intercept or attempt to intercept electronic mail not intended for you.
6. Advertise or offer to sell or buy any goods or services for any business purpose, unless such Community Feature specifically allows such messages.
7. Conduct or forward surveys, contests, pyramid schemes or chain letters.
8. Download any file posted by another user of a Community Feature that you know, or reasonably should know, cannot be legally distributed in such manner or that you have a contractual obligation to keep confidential (notwithstanding its availability on the Web Site).
9. Falsify or delete any author attributions, legal or other proper notices or proprietary designations or labels of the origin or source of software or other material contained in a file that is uploaded.
10. Misrepresent an affiliation with any person or organization.
11. Engage in any other conduct that restricts or inhibits anyone’s use of the Web Site, or which, as determined by the Company, may harm the Company or users of the Web Site or expose them to liability.
12. Violate any applicable laws or regulations or violate any code of conduct or other guidelines which may be applicable for any particular Community Feature .
13. Harvest or otherwise collect information about others, including e-mail addresses, without their consent.
B. You understand and acknowledge that you are responsible for whatever content you submit, you, not the Company, have full responsibility for such content, including its legality, reliability and appropriateness. If you post in the name of or on behalf of your employer or another entity, you represent and warrant that you are authorized to do so. By uploading or otherwise transmitting material to any area of the Web Site, you warrant that the material is your own or is in the public domain or otherwise free of proprietary or other restrictions and that you have the right to post it to the Web Site. Additionally, by uploading or otherwise transmitting material to any area of the Web Site you are granting the Company a irrevocable, royalty-free, worldwide right to publish, reproduce, use, adapt, edit and/or modify such material in any way, in any and all media now known or hereafter discovered, worldwide, including on the Internet and World Wide Web, for advertising, commercial, trade and promotional purposes, without additional limitation or compensation, unless prohibited by law, and without notice, review or approval.
C. The Company reserves the right, but does not assume any responsibility, to (1) remove any material posted on the Web Site which the Company, in its sole discretion, deems inconsistent with the foregoing commitments, or otherwise inappropriate for any reason; and (2) terminate any user’s access to all or part of the Web Site. However, the Company can neither review all material before it is posted on the Web Site nor ensure prompt removal of objectionable material after it has been posted. Accordingly, the Company assumes no liability for any action or inaction regarding transmissions, communications or content provided by third parties. The Company reserves the right to take any action it deems necessary to protect the personal safety of users of this Web Site and the public; however, the Company has no liability or responsibility to anyone for performance or nonperformance of the activities described in this paragraph.
D. Your failure to comply with the provisions of (A) or (B) above may result in the termination of your access to the Web Site and may expose you to civil and/or criminal liability.
Special note about Community Feature Content
Any content and/or opinions uploaded, expressed or submitted through any Community Feature or any other publicly available section of the Web Site (including password-protected areas), and all articles and responses to questions, other than the content explicitly authorized by the Company, are solely the opinions and responsibility of the person or entity submitting them and do not necessarily reflect the opinions of the Company. By way of example, any recommended or suggested use of products or services available from the Company that is posted through a Community Feature is not a sign of approval or recommendation by the Company. If you choose to follow any such recommendation you do so at your own risk.
Links to Third Party Sites
The Web Site may contain links to other websites on the internet. The Company is not responsible for the content, products, services or practices of any third party websites, including without limitation sites linked to or from the Web Site, sites framed within the Web Site or third party advertisements, and does not make representations regarding their quality, content or accuracy. The presence of links from the Web Site to any third party website does not mean that we approve of, endorse or recommend that website. We disclaim all warranties, express or implied, as to the accuracy, legality, reliability or validity of any content on any third party website. Your use of third party websites is at your own risk and subject to the terms and conditions of use for such websites.
Proprietary Rights to Content
You acknowledge and agree that the entire contents of the Web Site (including all information, data, software, graphics, text, images, logos, and/or other material) and the design, selection, collection, arrangement and assembly thereof, are proprietary to the Company and are protected by United States and international intellectual property laws. You are authorized only to use the content on the Web Site for personal use or legitimate business purposes. You may not copy, modify, create derivative works of, publicly display or perform, republish, store, transmit, distribute, remove, delete, augment, add to, participate in the transfer of, license or sell any of the material on the Web Site without the prior written consent of the Company, except to: (a) store copies of such materials temporarily in RAM, (b) store files that are automatically cached by your Web browser for display enhancement purposes, and (c) print a reasonable number of pages of a Web Site; provided in each case that you do not alter or remove any copyright or other proprietary notices included in such materials. Neither the title nor any intellectual property rights to any information or material on the Web Site are transferred to you, but remain with the Company or the applicable owner of such content.
Trademarks
The Company name and logo, and all related names, logos, product and service names appearing on the Web Site are trademarks of the Company and/or the applicable third party suppliers. They may not be used or redisplayed without the Company’s prior written consent.
Disclaimer of Liability
The Company does not assume any liability for the materials, information and opinions provided on, or available through, the Web Site (the “Site Content”). Reliance on the Site Content is solely at your own risk. The Company disclaims any liability for injury or damages resulting from the use of any Site Content.
THE WEB SITE, THE SITE CONTENT AND THE PRODUCTS AND SERVICES PROVIDED ON OR AVAILABLE THROUGH THE WEB SITE ARE PROVIDED ON AN “AS IS” AND “AS AVAILABLE” BASIS, WITH ALL FAULTS. NEITHER THE COMPANY NOR ANY PERSON ASSOCIATED WITH THE COMPANY MAKES ANY WARRANTY OR REPRESENTATION WITH RESPECT TO THE QUALITY, ACCURACY, OR AVAILABILITY OF THE WEB SITE. SPECIFICALLY, BUT WITHOUT LIMITING THE FOREGOING, NEITHER THE COMPANY NOR ANYONE ASSOCIATED WITH THE COMPANY WARRANTS OR REPRESENTS THAT THE WEB SITE, THE SITE CONTENT OR THE SERVICES PROVIDED ON OR THROUGH THE WEB SITE WILL BE ACCURATE, RELIABLE, ERROR-FREE OR UNINTERRUPTED; THAT DEFECTS WILL BE CORRECTED; THAT THE WEB SITE OR THE SERVER THAT MAKES IT AVAILABLE ARE FREE OF VIRUSES OR OTHER HARMFUL COMPONENTS; OR THAT THE WEB SITE WILL OTHERWISE MEET YOUR NEEDS OR EXPECTATIONS. THE COMPANY DISCLAIMS ALL WARRANTIES OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NON-INFRINGEMENT.
IN NO EVENT WILL THE COMPANY OR ITS LICENSORS OR CONTRACTORS BE LIABLE FOR ANY DAMAGES OF ANY KIND, UNDER ANY LEGAL THEORY, ARISING OUT OF OR IN CONNECTION WITH YOUR USE OF, OR INABILITY TO USE, THE WEB SITE, THE SITE CONTENT, ANY SERVICES PROVIDED ON OR THROUGH THE WEB SITE OR ANY LINKED SITE, INCLUDING ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, CONSEQUENTIAL OR PUNITIVE DAMAGES, INCLUDING, BUT NOT LIMITED TO, PERSONAL INJURY, LOST PROFITS OR DAMAGES RESULTING FROM DELAY, INTERRUPTION IN SERVICE, VIRUSES, DELETION OF FILES OR ELECTRONIC COMMUNICATIONS, OR ERRORS, OMISSIONS OR OTHER INACCURACIES IN THE WEB SITE OR THE SITE CONTENT OR SERVICES, WHETHER OR NOT THERE IS NEGLIGENCE BY THE COMPANY AND WHETHER OR NOT THE COMPANY HAS BEEN ADVISED OF THE POSSIBILITY OF ANY SUCH DAMAGES, UNLESS PROHIBITED BY APPLICABLE LAW.
Indemnification
You agree to indemnify and hold harmless the Company and its officers, directors, agents, employees, and others involved in the Web Site, from and against any and all liabilities, expenses, damages and costs, including reasonable attorneys’ fees, arising from any violation by you of these Terms and Conditions of Use, your use of the Web Site or any products, services or information obtained from or through the Web Site, your connection to the Web Site, any content you submit on the Web Site through any Community Feature , or your violation of any rights of another.
Applicable Law; International Use
These terms will be governed by and construed in accordance with the laws of the State of Pennsylvania, without regard to any principles of conflicts of law. You agree that any action at law or in equity that arises out of or relates to these Terms and Conditions of Use will be filed exclusively in the state or federal courts located in Pennsylvania and you hereby consent and submit to the personal jurisdiction of such courts for the purposes of litigating any such action.
These Terms and Conditions of Use are applicable to users in the United States, Canada, and Puerto Rico. If you are accessing the Web Site from outside the United States, Canada, or Puerto Rico, please see the appropriate international website, available at www.vwr.com, for applicable terms and conditions. If you elect to access this Web Site from outside of the specified jurisdictions rather than use the available international sites, you agree to these Terms and Conditions of Use and that such terms will be governed and construed with the laws of the United States and the State of Pennsylvania and that we make no representation that the materials or services on this Web Site are appropriate or available for use in those other jurisdictions. In any event, all users are responsible for their own compliance with local laws.
General Terms
These Terms and Conditions of Use, as they may be amended from time to time, constitute the entire agreement and understanding between you and us governing your use of the Web Site. Our failure to exercise or enforce any right or provision of the Terms and Conditions of Use shall not constitute a waiver of such right or provision. If any provision of the Terms and Conditions of Use is found by a court of competent jurisdiction to be invalid, you nevertheless agree that the court should endeavor to give effect to the parties' intentions as reflected in the provision, and the other provisions of the Terms and Conditions of Use shall remain in full force and effect. Neither a course of dealing or conduct between you and the Company nor any trade practices shall be deemed to modify these Terms and Conditions of Use. You agree that regardless of any statute or law to the contrary, any claim or cause of action arising out of or related to use of the Site or the Terms and Conditions of Use must be filed within one (1) year after such claim or cause of action arose or be forever barred. Any rights not expressly granted herein are reserved by and for the Company. We may terminate your access, or suspend any user's access to all or part of the Site, without notice, for any conduct that we, in our sole discretion, believe is in violation of any applicable law or is harmful to the interests of another user, a third-party provider, a service provider, or us. Any inquiries concerning these Terms and Conditions of Use should be directed to solutions@vwr.com.
Copyright Complaints
We respect the intellectual property of others, and we ask our users to do the same. If you believe that your work has been copied and is accessible on the Site in a way that constitutes copyright infringement, you may notify us by providing our copyright agent the following information:
an electronic or physical signature of the person authorized to act on behalf of the owner of the copyright interest;
a description of the copyrighted work that your claim has been infringed;
identification of the URL or other specific location on the Site where the material that you claim is infringing is located;
your address, telephone number, and e-mail address;
a statement by you that you have a good faith belief that the disputed use is not authorized by the copyright owner, its agent, or the law; and
a statement by you, made under penalty of perjury, that the above information in your notice is accurate and that you are the copyright owner or authorized to act on the copyright owner's behalf.
Our agent for notice of claims of copyright infringement on the Site can be reached at: solutions@vwr.com.
FAQs
What is the minimum essential medium? ›
Minimum Essential Medium (MEM) contains a higher concentration of amino acids than Basal Medium Eagle (BME). MEM media may be formulated with Earle's salts for maintenance of pH in a CO2 incubator, or Hanks' salts for maintenance of pH in atmospheric conditions.
What is the Eagle's minimum essential medium? ›Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation.
What is EMEM in cell culture? ›Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations.
What does EMEM contain? ›Eagle's minimum essential medium modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.
Is EMEM the same as MEM? ›A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
What is modified minimum essential medium? ›MEM-Eagle — A Modified Minimum Essential Medium (MEM)
MEM is one of the most used cell culture media and can be used with a variety of suspension and adherent mammalian cells, including HeLa, fibroblasts and primary rat astrocytes.
The key difference between two media is the nutrient composition. EMEM is composed of the minimum concentrations of nutrients required for the growth of the culture, whereas DMEM is a much complex media with increased concentrations of amino acids and vitamins.
What is the difference between eagle's minimum essential medium and DMEM? ›DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
What is eagle's medium used for? ›Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
What is EMEM used for? ›EMEM (Eagle's Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture.
What is minimum essential medium cell culture? ›
Gibco Minimum Essential Media (MEM), patterned after Eagle's Media, is one of the most commonly used of all cell culture media and is well suited for the growth of a broad spectrum of mammalian cells. Compared to the earlier Basal Medium Eagle (BME), MEM is formulated with increased concentrations of amino acids.
What is the full form of EMEM media? ›eagle's minimum essential medium (emem)
What is the composition of modified Eagle's medium? ›Dulbecco's Modified Eagle's Medium (DMEM) is modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability.
What is the difference between DMEM and AMEM? ›Background: Alpha modified Eagle's minimum essential medium (α-MEM) and Dulbecco's modified Eagle's medium (DMEM) are the basic media for cell culture. α-MEM has minimum glucose and amino acid concentration than DMEM.
Does EMEM have phenol red? ›MEM, no glutamine, no phenol red.
Does MEM contain calcium? ›Minimum Essential Medium Eagle (MEM) (Joklik Modification) (For Suspension Culture) w/ L-Glutamine w/o Calcium chloride and Sodium bicarbonate. AT079 is Joklik modification of Minimum Essential Medium. This medium is designed for use with suspension cells, hence does not contain calcium.
What does minimal essential medium not contain? ›AL104 is Minimum Essential Medium Eagle with Earle's salts and non-essential amino acids. It does not contain L-glutamine and sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
What is minimum essential product? ›A minimum viable product (MVP) is a version of a product with just enough features to be usable by early customers who can then provide feedback for future product development. A focus on releasing an MVP means that developers potentially avoid lengthy and (possibly) unnecessary work.
Can we use DMEM instead of EMEM? ›Both mediums lack protein and contain the amino acids, salts, glucose and vitamins, required to provide a cell with energy and maintain cells in tissue culture, but the DMEM formulation is modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM.
Can you use RPMI instead of DMEM? ›DMEM is used in culturing cells in adherent cultures. The key difference between the two media is the type of culture of the media. RPMI is used on suspension cultures, whereas DMEM is used to culture adherent cells.
Can bacteria grow in DMEM? ›
example, growth in DMEM ( Fig 1A) and YESCA broth ( Fig 1B) at 28°C resulted in doubling times of about 240 min and 180 min, respectively, for all bacterial strains.
What does Eagle's MEM contain? ›- Bone Morphogenetic Protein.
- Streptomycin.
- Fetal Bovine Serum.
- Cell Culture.
- Amino Acids.
- Lysine.
- Cell Line.
DMEM is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DMEM formula composition contains 1000 mg/L of glucose and was first reported for culturing embryonic mouse cells.
Is DMEM a complete medium? ›DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.
Why is the eagle used so much? ›The eagle is used in heraldry as a charge, as a supporter, and as a crest. Heraldic eagles can be found throughout world history like in the Achaemenid Empire or in the present Republic of Indonesia.
What is eagle's minimum essential medium f12? ›DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
What is eagle's minimum essential medium with Earle's salts? ›Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells.
What are the different types of MEM media? ›Today there are many other MEM formulations available, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.
What are the 5 main components of cell culture base media? ›Essential components of cell culture media include carbohydrates, amino acids, vitamins, minerals, and a pH buffer system. Your choice of media can affect the accuracy, reproducibility, and relevance of your experimental results.
What are the three types of cell culture media? ›Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.
What does MS media in plant tissue culture media stand for? ›
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MS medium was originally formulated by Murashige and Skoog in 1962 to optimize tobacco callus bioassay system for facilitating the study of cytokinins.
What is the DMEM medium called? ›Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.
What is Alpha MEM media? ›MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells.
Can you drink cell media? ›My summer UROP involved some cell culture training, which started off with “Try not to drink the media.” This really just implies that cell media is, in fact, drinkable.
What is the difference between DMEM and BME? ›In comparison with Eagle's BME, DMEM contains a four-fold higher concentration of amino acids and vitamins as well as additional nutrient components. DMEM is typically supplemented with fetal bovine serum or uses serum-free supplements.
How do you make DMEM media from powder? ›- Prepare 900ml of distilled water in clean glass beaker. ...
- Add the 1-liter powder to the water and stir gently. ...
- Add 3.7 gram Sodium Bicarbonate (or 49.4 ml of 7.5% Sodium Bicarbonate Solution).
- Adjust pH to 0.2-0.3 units below the required pH using 1N HCl or 1N NaOH.
Eagle's minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations.
What is the difference between DMEM and IMDM medium? ›IMDM is a modification of DMEM containing selenium, and has additional amino acids, vitamins and inorganic salts as compared to DMEM. It has potassium nitrate instead of ferric nitrate and also contains HEPES and sodium pyruvate.
Can I use RPMI for adherent cells? ›A modification of McCoy′s 5A medium (or RPMI 1630), it was originally formulated to support lymphoblastoid cells in suspension cultures, but can also support a wide variety of adherent cells.
What is EMEM media without phenol red? ›EMEM (Eagle's Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. EMEM has a higher amino acid concentration and 2 to 5 times greater vitamin concentration than BME, permitting longer periods between feedings.
What is the difference between methylene blue and phenol red? ›
Methylene blue is an ionic compound containing a colored, positively charged organic dye molecule and a chloride counterion. Phenol red is a colored organic dye that acts as an acid–base indicator. At pH 10, phenol red carries a double negative charge.
Does phenol red affect cell growth? ›We observed that phenol red added to the culture medium strongly promoted the cell proliferation and cell cycle progression of human cells expressing the estrogen receptor, and affected their susceptibility to chemotherapy.
What is minimum medium culture? ›Minimal media are those that contain the minimum nutrients possible for colony growth, generally without the presence of amino acids, and are often used by microbiologists and geneticists to grow "wild-type" microorganisms.
What is minimum essential medium α? ›MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid.
What is minimum essential medium Earle's? ›Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells. MEM is formulated with either Earle's salts, Hanks' salts or alpha-modified.
What is the difference between Eagle's minimal essential medium and DMEM? ›DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
What are examples of minimal medium? ›Minimal media is a defined medium with the composition varying on the microorganism being cultured. Typically, this medium contains a carbon source such as a sugar/succinate, various inorganic salts (salts of essential elements like magnesium, nitrogen, phosphorous and sulphur) and water.
What does minimal medium mean in biology? ›Minimal media – Media that contains the minimum nutrients possible for colony growth, generally without the presence of amino acids, and are often used by microbiologists and geneticists to grow “wild type” microorganisms. These media can also be used to select for or against the growth of specific microbes.
What is the difference between medium and minimum? ›What is the difference between minimum and medium security prisons? Medium security prisons typically guard incarcerated people more closely than those in minimum security. People housed in a minimum security prison are often allowed to leave the prison during the day to work in the community.
What is MEC and minimum value? ›The Minimum Value refers to the comprehensiveness that a plan offers. An MEC, for instance, has a MV of 60 percent, which is the Actuarial Value. This means that an MEC pays a minimum of 60 percent of all benefits provided in the health plan in terms of actuarial value.
What is minimum essential value? ›
Minimum Essential Coverage (MEC) and Minimum Value (MV): Minimum Essential Coverage is a lower threshold than Minimum Value (MV). MV is the 60% Actuarial Value and is met when a plan pays on average at least 60% of the actuarial value of allowed benefits under the plan.
What is Eagle's minimum essential medium with Earle's salts? ›Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells.
What is in minimal basal medium? ›Basal media contain amino acids, glucose, and ions (calcium, magnesium, potassium, sodium, and phosphate) essential for cell survival and growth.
What does Earle's balanced salt solution do? ›Earle's Balanced Salt Solution (EBSS) is designed for the short–term maintenance of cells in a CO2 environment. EBSS is commonly used for different cell culture applications such as washing, transporting, and dilution.
What is Eagle's medium used for? ›Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.