CCL-30™
RPMI 2650 is a cell line exhibiting epithelial morphology from the nasal septum of a 52-year-old, male patient with squamous cell carcinoma. This cell line was deposited by GE Moore and can be used in cancer research, and toxicology.
- Product category
-
Human cells
- Organism
-
Homo sapiens, human
- Morphology
- epithelial
- Tissue
- Nose; Nasal septum
- Disease
-
Squamous Cell Carcinoma
- Applications
-
3D cell culture
Cancer research
High-throughput screening
Toxicology
- Product format
- Frozen
- Storage conditions
-
Vapor phase of liquid nitrogen
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Documentation
Certificate of Analysis Download
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Certificate of Origin Download
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Certificate of Origin Request
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You can find your account number on your sales order confirmation or order invoice.
This product sheet is not available online. We only provide this product sheet to customers who have purchased this biosafety level 3 product. If you purchased this product, please contact the distributor from whom you purchased this product for this product sheet.
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Please check the Product Sheet and Safety Data Sheet Landing pagefor more information.
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BSL 1 ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Eagle's Minimum Essential Medium (EMEM)
30-2003
Fetal Bovine Serum (FBS)
30-2020
Dimethylsulfoxide (DMSO)
4-X
Detailed product information
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Characteristics
- Growth properties
- Adherent
- Age
- 52 years
- Gender
- Male
- Metastatic
- Pleural effusion
- Virus susceptibility
-
Human poliovirus 1
Herpes simplex virus
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
- Genes expressed
- mucoid; keratin
- Isoenzymes
-
G6PD, B
- Comments
-
The cells are positive for keratin by immunoperoxidase staining.
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
- Handling procedure
-
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
- Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
- Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 125 x g for 5 to 7 minutes.
- Resuspend cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
- Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
- Subculturing procedure
-
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
NOTE: Cells attach in clusters. Cells will pile and the culture does not get 100% confluent.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Quality control specifications
- Mycoplasma contamination
- Not detected
- STR profiling
-
Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18
History
- Deposited as
- Homo sapiens
- Depositors
- GE Moore
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Export Certificate of Origin
Customers from Argentina, Columbia, Egypt, Ethiopia, Germany, Greece, India, Jordan, Lebanon, Peru, Qatar, Saudi Arabia, Spain, and United Arab Emirates
This material may require an export certificate of origin acquired by either ATCC or a forwarding agent; you do not need to take any action for this export certificate of origin. Additional fees may apply as a result of acquiring this export certificate of origin; these fees will be applied after your order is confirmed and our Customer Care team reaches out about this item. We cannot ship this item until we acquire this export certificate of origin. The export certificate of origin will be included in the shipment to meet export requirements. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.
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Frequently Asked Questions
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FAQs
What is RPMI 2650 cell culture? ›
RPMI 2650 is a cell line exhibiting epithelial morphology from the nasal septum of a 52-year-old, male patient with squamous cell carcinoma. This cell line was deposited by GE Moore and can be used in cancer research, and toxicology.
What is Caco 2 cell line? ›The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine.
How do you culture huh7 cells? ›HuH-7 Cells should be cultured in DMEM supplemented with 10% FBS. Cells should be grown at 37 °C in a 5% CO2 setting.
What does RPMI stand for? ›Roswell Park Memorial Institute (RPMI) medium or RPMI 1640 is a form of medium used in cell culture and tissue culture. It has been used for growing a variety of mammalian cell lines and growth of human lymphocytes.
What does RPMI agar stand for? ›RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966.
What are the three types of cell lines? ›The Cell Line
Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.
The medium normally used for differentiation of Caco-2 cells contains a supplement of foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) compartments.
What are the two types of cell lines? ›Cell lines can be finite or continuous. An immortalized or continuous cell line has acquired the ability to proliferate indefinitely, either through genetic mutations or artificial modifications. A finite cell line has been sub-cultured for 20-80 passages after which they senesce.
What does HuH-7 cells stand for? ›HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
How do you culture C2C12 cells? ›C2C12 cells are cultured in growth medium in collagen-coated 150 cm2 polystyrene flasks at 37 °C in humidified atmosphere of 5% CO2. CRITICAL STEP: To avoid premature myoblast differentiation, cells are kept at very low confluence (~ 40%) and propagated before they reach higher confluency.
Why use HuH-7 cells? ›
Advantages of HuH7 cells
HuH-7 cells secrete a growth factor that helps cells grow without serum. Transfection flexibility: HuH7 cell line is widely used for transfection purposes due to high receptiveness for the HCV genome. Therefore, these cells are pivotal for anti-HCV drug screening and development.
2 ) Why is it pink/red? It has a phenol red pH indicator that will turn from pink to yellow indicating contamination or exposure to air. Any shade of pink/reddish-orange is acceptable. The exact color varies from lot to lot and vendor to vendor, so another lab's supplied RPMI may not be exactly the same color.
How long is tissue good in RPMI? ›Specimens are stable up to 24 hours at 2-8ºC in RPMI.
How long does RPMI last? ›RPMI 1640 has a shelf life of 18-24 months, depending on formulation.
Can bacteria grow in RPMI? ›An RPMI medium solution is known to sustain growth of bacteria and fungi, and it is not needed for transport of tissue specimens to the microbiology laboratory.
What is the difference between RPMI and advanced RPMI? ›Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.
What is RPMI made of? ›One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
What is the most famous cell line? ›The HeLa cell line is the first and also the most famous immortal cell line. This cell line is named after Henrietta Lacks, an Afri- can-American woman that died of cancer on October 4, 1951. In February 1951, cervical cancer cells were taken from her and put into culture.
What is the most used cell line? ›HeLa cells are the world's most commonly used human cell lines, and have served as a standard for understanding many fundamental biological processes.
What is the best cell line? ›HEK293, or human embryonic kidney-derived epithelial cells, are arguably one of the most commonly used cell lines in cell biology research. But why?
What is the most common cell culture media? ›
Serum. Serum is a complex mix of albumins, growth factors and growth inhibitors and is probably one of the most important components of cell culture medium. The most commonly used serum is fetal bovine serum (FBS). Other types of serum are available including newborn calf serum and horse serum.
Which DMSO for cell culture? ›0.5% DMSO as the final concentration has been used widely for cell culture without cytotoxicity. 1% DMSO doesn't cause any toxicity to some cells but 0.5% DMSO is recommended.
Why are Caco-2 cells used? ›The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine.
Can I use RPMI instead of DMEM? ›A: For some adherent cell lines we strongly recommend using RPMI instead of DMEM medium.
What is the difference between RPMI and DMEM? ›RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is a modified type of basal medium, with increased amino acid and vitamin concentrations up to a fourfold. DMEM is used in culturing cells in adherent cultures.
How long can cells survive without CO2? ›Optimize the sample environment. Mammalian cells in bicarbonate-based media require 5% CO2 to maintain physiological pH. Without a CO2 supply, cells are adversely affected within five minutes.
What are Phoenix cells? ›Phoenix-AMPHO is an epithelial-like cell that was isolated from the kidney of a patient. This cell line was deposited by G Nolan in 1996 and can be used in vaccine development.
What are H4 cells? ›H4 is an epithelial cell line that was isolated from the brain of a 37-year-old, White male with neuroglioma. The cell line H4 can be used in neuroscience research.
What are A10 cells? ›Objectives: The A10 cell line was derived from the thoracic aorta of embryonic rat and is a commonly used model of vascular smooth muscle cells (VSMC). Despite its wide use this cell line has not been well characterized.
What do C2C12 cells do? ›C2C12 cells are used to study the differentiation of myoblasts, osteoblasts, and myogenesis, to express various target proteins, and to explore mechanistic biochemical pathways.
What is L6 cells? ›
L6 is a myoblast cell isolated from the skeletal muscle from a rat. This cell line was deposited by D Schubert.
What are cos 7 cells used for? ›The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids.
What type of cells are HuH-7? ›HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.
Why use THP 1 cells? ›THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities.
What is in RPMI media? ›RPMI composition
RPMI 1640 Medium contains the reducing agent glutathione as well as biotin, vitamin B12, and PABA, which are vitamins not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, RPMI 1640 media includes high concentrations of the vitamins inositol and choline.
RPMI is a media used widely to culture mammalian cells in suspension culture. DMEM is a modified type of basal medium, with increased amino acid and vitamin concentrations up to a fourfold. DMEM is used in culturing cells in adherent cultures.
What is RPMI media made of? ›One liter of RPMI 1640 contains: Glucose (2 g) pH indicator (phenol red, 5 mg) Salts (6 g sodium chloride, 2 g sodium bicarbonate, 1.512 g disodium phosphate, 400 mg potassium chloride, 100 mg magnesium sulfate, and 100 mg calcium nitrate)
What is the difference between RPMI and RPMI 1640? ›Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.
Can you drink cell media? ›My summer UROP involved some cell culture training, which started off with “Try not to drink the media.” This really just implies that cell media is, in fact, drinkable.
What are the three types of cell culture media? ›Cells cultured in the lab can be classified into three different types: primary cells, transformed cells, and self-renewing cells.
What are the three basic classes of media in cell culture? ›
The three basic classes of media are basal media, reduced-serum media, and serum-free media, which differ in their requirement for supplementation with serum.
What temperature should RPMI be stored at? ›Storage/Stability
Store the dry powdered medium at 2-8°C under dry conditions and liquid medium at 2-8°C in the dark.
- Prepare 900ml of distilled water in clean glass beaker. ...
- Add the 1-liter powder to the water and stir gently. ...
- Add 2 gram Sodium Bicarbonate (or 26.67 ml of 7.5% Sodium Bicarbonate Solution).
- Adjust pH to 0.2-0.3 units below the required pH using 1N HCl or 1N NaOH.
RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.
What is the alternative to RPMI 1640? ›Müeller-Hinton methylene blue media as an alternative to RPMI 1640 for determining the susceptibility of Cryptococcus neoformans and Cryptococcus gattii to posaconazole with Etest. Mycoses.
What does DMEM stand for? ›DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Does RPMI media contain glucose? ›RPMI 1640 Medium, no glucose.